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Text File  |  1993-02-27  |  28KB  |  403 lines
  1. REBASE version 9206
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  7.                Dr. Richard J. Roberts Restriction Enzyme Database
  8.                 Copyright (c) Cold Spring Harbor Laboratory 1992
  9.                               All rights reserved.
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  18. Rich Roberts                                                        May 29 92
  19.  
  20. REBASE abbreviations for commercial sources of restriction enzymes & methylases
  21.  
  22.         A    Amersham (12/91)
  23.         B    BRL (4/92)
  24.         E    Stratagene (4/92)
  25.         F    Fermentas (6/91)
  26.         H    American Allied (4/92)
  27.         I    IBI (6/91)
  28.         K    Takara (4/92)
  29.         L    Northumbria Biologicals Ltd. (10/91)
  30.         M    Boehringer Mannheim (9/91)
  31.         N    New England Biolabs (4/92)
  32.         O    Toyobo (4/92)
  33.         P    PL-Pharmacia-LKB (9/91)
  34.         Q    Molecular Biology Resources (4/92)
  35.         R    Promega Corporation (10/91)
  36.         S    Sigma (4/92)
  37.         U    USB (4/92)
  38.         V    Serva (2/91)
  39.         W    ILS (4/92)
  40.         X    New York Biolabs (4/92)
  41.  
  42.  
  43.  
  44. ..
  45. ;AatI      3 AGG'CCT        0 !  Eco147I,StuI                      >OU
  46. AatII      5 G_ACGT'C      -4 !                                    >ELMNOPRSUVX
  47. AccI       2 GT'mk_AC       2 !                                    >ABEIKLMNOPQRSUVX
  48. ;AccII     2 CG'CG          0 !  Bsp50I,BstUI,MvnI,ThaI            >AKQVX
  49. ;AccIII    1 T'CCGG_A       4 !  BseAI,BsiMI,BspEI,Kpn2I,MroI      >AEKQRV
  50. ;Acc65I    1 G'GTAC_C       4 !  Asp718I,KpnI                      >FNR
  51. AciI       1 C'CGC          4 !                                    >N
  52. ;AcyI      2 Gr'CG_yC       2 !  BbiII,BsaHI,Hin1I                 >MRV
  53. ;AfaI      2 GT'AC          0 !  Csp6I,RsaI                        >K
  54. AflII      1 C'TTAA_G       4 !  BfrI                              >ABKNSU
  55. AflIII     1 A'CryG_T       4 !                                    >BMNU
  56. AgeI       1 A'CCGG_T       4 !                                    >N
  57. ;AhaIII    3 TTT'AAA        0 !  DraI                              >
  58. AluI       2 AG'CT          0 !                                    >ABEFHIKLMNOPQRSUVX
  59. AlwI       9 GGATCnnnn'n_   1 !                                    >NSU
  60. ;Alw21I    5 G_wGCw'C      -4 !  AspHI,BsiHKAI,HgiAI               >F
  61. ;Alw26I    6 GTCTCn'nnnn_   4 !  BsmAI                             >FR
  62. ;Alw44I    1 G'TGCA_C       4 !  ApaLI,SnoI                        >BFORU
  63. AlwNI      6 CAG_nnn'CTG   -3 !                                    >BNSU
  64. ;Aor51HI   3 AGC'GCT        0 !  Eco47III                          >K
  65. ApaI       5 G_GGCC'C      -4 !  Bsp120I                           >BEIKLMNOPRSUVX
  66. ApaBI      8 GCA_nnnnn'TGC  -5 !                                    >
  67. ApaLI      1 G'TGCA_C       4 !  Alw44I,SnoI                       >AEKNSUX
  68. ApoI       1 r'AATT_y       4 !                                    >N
  69. ;ApyI      2 CC'w_GG        1 !  BsiLI,BstNI,BstOI,EcoRII,MvaI     >M
  70. AscI       2 GG'CGCG_CC     4 !                                    >N
  71. AseI       2 AT'TA_AT       2 !  AsnI,VspI                         >NSU
  72. ;AsnI      2 AT'TA_AT       2 !  AseI,VspI                         >M
  73. ;AspI      4 GACn'n_nGTC    1 !  Tth111I                           >M
  74. ;Asp700I   5 GAAnn'nnTTC    0 !  XmnI                              >M
  75. ;Asp718I   1 G'GTAC_C       4 !  Acc65I,KpnI                       >M
  76. ;AspHI     5 G_wGCw'C      -4 !  Alw21I,BsiHKAI,HgiAI              >M
  77. ;AsuI      1 G'GnC_C        3 !  BsiZI,Cfr13I,Sau96I               >R
  78. ;AsuII     2 TT'CG_AA       2 !  BsiCI,Bsp119I,BstBI,Csp45I,LspI,NspV,SfuI  >
  79. AvaI       1 C'yCGr_G       4 !  BcoI,Eco88I,NspIII                >ABEIKLMNOPRSUVX
  80. AvaII      1 G'GwC_C        3 !  Eco47I,SinI                       >ABEIKMNPRSX
  81. ;AvaIII    3 ATGCAT         ? !  EcoT22I,Mph1103I,NsiI,Ppu10I      >
  82. ;AviII     3 TGC'GCA        0 !  FdiII,FspI,MstI                   >M
  83. AvrII      1 C'CTAG_G       4 !  BlnI                              >N
  84. ;AxyI      2 CC'TnA_GG      3 !  Bsu36I,CvnI,Eco81I,MstII,SauI     >V
  85. BaeI       5 ACnnnnGTAyC    ? !                                    >
  86. ;BalI      3 TGG'CCA        0 !  MscI                              >AIKRSVX
  87. BamHI      1 G'GATC_C       4 !  BstI                              >ABEFHIKLMNOPQRSUVWX
  88. BanI       1 G'GyrC_C       4 !  Eco64I                            >EIMNOPRSUVX
  89. BanII      5 G_rGCy'C      -4 !  Eco24I                            >BEIKLMNOPRSUVX
  90. ;BanIII    2 AT'CG_AT       2 !  BscI,BsiXI,Bsp106I,BspDI,Bsu15I,ClaI  >OU
  91. ;BbeI      5 G_GCGC'C      -4 !  EheI,KasI,NarI                    >AK
  92. ;BbiII     2 Gr'CG_yC       2 !  AcyI,BsaHI,Hin1I                  >AK
  93. ;BbrPI     3 CAC'GTG        0 !  Eco72I,PmaCI,PmlI                 >M
  94. BbsI       8 GAAGACnn'nnnn_   4 !                                    >NU
  95. ;BbuI      5 G_CATG'C      -4 !  PaeI,SphI                         >RV
  96. BbvI      13 GCAGCnnnnnnnn'nnnn_   4 !  Bst71I                            >EINX
  97. ;BbvII     8 GAAGACnn'nnnn_   4 !  BbsI                              >
  98. BccI       2 CCATC          ? !                                    >
  99. Bce83I    22 CTTGAGnnnnnnnnnnnnnn_nn'  -2 !                                    >
  100. BcefI     17 ACGGCnnnnnnnnnnnn'n_   1 !                                    >
  101. BcgI      24 CGAnnnnnnTGCnnnnnnnnnn_nn'  -2 !                                    >NU
  102. BcgI      24 GCAnnnnnnTCGnnnnnnnnnn_nn'  -2 !                                    >NU
  103. BclI       1 T'GATC_A       4 !  BsiQI,FbaI                        >ABEFILMNOPRSUVX
  104. ;BcnI      2 CC's_GG        1 !  NciI                              >AFK
  105. ;BcoI      1 C'yCGr_G       4 !  AvaI,Eco88I,NspIII                >AW
  106. ;BetI      1 w'CCGG_w       4 !  BsaWI                             >
  107. BfaI       1 C'TA_G         2 !  MaeI,RmaI                         >N
  108. ;BfrI      1 C'TTAA_G       4 !  AflII                             >M
  109. BglI       7 GCCn_nnn'nGGC  -3 !                                    >ABEFHILMNOPQRSUVWX
  110. BglII      1 A'GATC_T       4 !                                    >ABEFHIKLMNOPQRSUVWX
  111. ;BinI      9 GGATCnnnn'n_   1 !  AlwI                              >
  112. ;BlnI      1 C'CTAG_G       4 !  AvrII                             >AK
  113. ;BmyI      5 G_dGCh'C      -4 !  Bsp1286I,SduI                     >M
  114. BpmI      22 CTGGAGnnnnnnnnnnnnnn_nn'  -2 !  GsuI                              >N
  115. Bpu10I     2 CC'TnAGC       7 !                                    >
  116. Bpu1102I   2 GC'TnA_GC      3 !  CelII,EspI                        >EFN
  117. BsaI       7 GGTCTCn'nnnn_   4 !  Eco31I                            >NU
  118. BsaAI      3 yAC'GTr        0 !                                    >NU
  119. BsaBI      5 GATnn'nnATC    0 !  BsiBI,MamI                        >NU
  120. BsaHI      2 Gr'CG_yC       2 !  AcyI,BbiII,Hin1I                  >NS
  121. BsaJI      1 C'CnnG_G       4 !                                    >NU
  122. BsaWI      3 wCCGGw         ? !                                    >N
  123. ;BscI      2 AT'CG_AT       2 !  BanIII,BsiXI,Bsp106I,BspDI,Bsu15I,ClaI  >L
  124. ;BscBI     3 GGn'nCC        0 !  NlaIV                             >AI
  125. ;BscCI     7 GAATG_Cn'     -2 !  BsmI                              >W
  126. BscGI      2 CCCGT          ? !                                    >
  127. ;BseAI     1 T'CCGG_A       4 !  AccIII,BsiMI,BspEI,Kpn2I,MroI     >M
  128. ;BsePI     3 GCGCGC         ? !  BssHII                            >
  129. BsgI      22 GTGCAGnnnnnnnnnnnnnn_nn'  -2 !                                    >N
  130. ;BshI      2 GG'CC          0 !  BsuRI,HaeIII,PalI                 >W
  131. BsiI       1 C'TCGTG        6 !                                    >
  132. ;BsiBI     5 GATnn'nnATC    0 !  BsaBI,MamI                        >AW
  133. ;BsiCI     2 TT'CG_AA       2 !  Bsp119I,BstBI,Csp45I,LspI,NspV,SfuI  >AUW
  134. BsiEI      4 CG_ry'CG      -2 !  McrI                              >ANW
  135. ;BsiHKAI   5 G_wGCw'C      -4 !  Alw21I,AspHI,HgiAI                >N
  136. ;BsiLI     2 CC'w_GG        1 !  ApyI,BstNI,BstOI,EcoRII,MvaI      >AW
  137. ;BsiMI     1 T'CCGG_A       4 !  AccIII,BseAI,BspEI,Kpn2I,MroI     >AW
  138. ;BsiQI     1 T'GATC_A       4 !  BclI,FbaI                         >AW
  139. ;BsiWI     1 C'GTAC_G       4 !  Pfl23II,SplI,SunI                 >AMNUW
  140. ;BsiXI     2 AT'CG_AT       2 !  BanIII,BscI,Bsp106I,BspDI,Bsu15I,ClaI  >AW
  141. ;BsiYI     7 CCnn_nnn'nnGG  -3 !  BslI                              >AMUW
  142. ;BsiZI     1 G'GnC_C        3 !  AsuI,Cfr13I,Sau96I                >AW
  143. BslI       7 CCnn_nnn'nnGG  -3 !  BsiYI                             >N
  144. BsmI       7 GAATG_Cn'     -2 !  BscCI                             >ELMNOSUVX
  145. BsmAI      6 GTCTCn'nnnn_   4 !  Alw26I                            >NU
  146. ;Bsp50I    2 CG'CG          0 !  AccII,BstUI,MvnI,ThaI             >E
  147. ;Bsp68I    3 TCG'CGA        0 !  NruI,SpoI                         >F
  148. ;Bsp106I   2 AT'CG_AT       2 !  BanIII,BscI,BsiXI,BspDI,Bsu15I,ClaI  >E
  149. ;Bsp119I   2 TT'CG_AA       2 !  BsiCI,BstBI,Csp45I,LspI,NspV,SfuI  >F
  150. ;Bsp120I   1 G'GGCC_C       4 !  ApaI                              >F
  151. ;Bsp143I   0 'GATC_         4 !  DpnII,MboI,NdeII,Sau3AI           >F
  152. ;Bsp143II   5 r_GCGC'y      -4 !  HaeII                             >F
  153. Bsp1286I   5 G_dGCh'C      -4 !  BmyI,SduI                         >AKNRUX
  154. ;BspCI     4 CG_AT'CG      -2 !  PvuI,XorII                        >E
  155. ;BspDI     2 AT'CG_AT       2 !  BanIII,BscI,BsiXI,Bsp106I,Bsu15I,ClaI  >N
  156. BspEI      1 T'CCGG_A       4 !  AccIII,BseAI,BsiMI,Kpn2I,MroI     >NS
  157. BspGI      3 CTGGAC         ? !                                    >
  158. BspHI      1 T'CATG_A       4 !                                    >NSU
  159. BspMI     10 ACCTGCnnnn'nnnn_   4 !                                    >NU
  160. ;BspMII    1 T'CCGG_A       4 !  AccIII,BseAI,BsiMI,BspEI,Kpn2I,MroI  >
  161. BspWI      7 GCnn_nnn'nnGC  -3 !                                    >M
  162. BsrI       6 ACTG_Gn'      -2 !                                    >NU
  163. BsrBI      3 GAG'CGG        6 !                                    >N
  164. BsrFI      1 r'CCGG_y       4 !  Cfr10I                            >N
  165. BssHII     1 G'CGCG_C       4 !                                    >BELMNOQRUVX
  166. ;BstI      1 G'GATC_C       4 !  BamHI                             >P
  167. ;Bst71I   13 GCAGCnnnnnnnn'nnnn_   4 !  BbvI                              >R
  168. Bst1107I   3 GTA'TAC        0 !                                    >FKMN
  169. ;BstBI     2 TT'CG_AA       2 !  BsiCI,Bsp119I,Csp45I,LspI,NspV,SfuI  >NS
  170. BstEII     1 G'GTnAC_C      5 !  BstPI,Eco91I,EcoO65I              >BEHLMNOPQRSUVX
  171. ;BstNI     2 CC'w_GG        1 !  ApyI,BsiLI,BstOI,EcoRII,MvaI      >ENSX
  172. ;BstOI     2 CC'w_GG        1 !  ApyI,BsiLI,BstNI,EcoRII,MvaI      >R
  173. ;BstPI     1 G'GTnAC_C      5 !  BstEII,Eco91I,EcoO65I             >K
  174. ;BstUI     2 CG'CG          0 !  AccII,Bsp50I,MvnI,ThaI            >NSU
  175. BstXI      8 CCAn_nnnn'nTGG  -4 !                                    >BEKLMNOQRSUVX
  176. BstYI      1 r'GATC_y       4 !  MflI,XhoII                        >BNU
  177. ;BstZI     1 C'GGCC_G       4 !  EagI,EclXI,Eco52I,XmaIII          >R
  178. ;Bsu15I    2 AT'CG_AT       2 !  BanIII,BscI,BsiXI,Bsp106I,BspDI,ClaI  >F
  179. Bsu36I     2 CC'TnA_GG      3 !  AxyI,CvnI,Eco81I,MstII,SauI       >NRS
  180. ;BsuRI     2 GG'CC          0 !  BshI,HaeIII,PalI                  >F
  181. Cac8I      3 GCn'nGC        0 !                                    >
  182. ;CauII     2 CC's_GG        1 !  BcnI,NciI                         >
  183. ;CcrI      1 C'TCGA_G       4 !  PaeR7I,XhoI                       >X
  184. ;CelII     2 GC'TnA_GC      3 !  Bpu1102I,EspI                     >ALM
  185. ;CfoI      3 G_CG'C        -2 !  HhaI,Hin6I,HinP1I                 >BILMRSV
  186. ;CfrI      1 y'GGCC_r       4 !  EaeI                              >
  187. ;Cfr9I     1 C'CCGG_G       4 !  PspAI,SmaI,XmaI                   >FOU
  188. ;Cfr10I    1 r'CCGG_y       4 !  BsrFI                             >AFKMOU
  189. ;Cfr13I    1 G'GnC_C        3 !  AsuI,BsiZI,Sau96I                 >FKOU
  190. ;Cfr42I    4 CC_GC'GG      -2 !  KspI,SacII,SstII                  >F
  191. ClaI       2 AT'CG_AT       2 !  BanIII,BscI,BsiXI,Bsp106I,BspDI,Bsu15I  >ABKMNPQRSVWX
  192. ;CpoI      2 CG'GwC_CG      3 !  CspI,RsrII                        >AFK
  193. ;CspI      2 CG'GwC_CG      3 !  CpoI,RsrII                        >EORV
  194. ;Csp6I     1 G'TA_C         2 !  AfaI,RsaI                         >F
  195. ;Csp45I    2 TT'CG_AA       2 !  BsiCI,Bsp119I,BstBI,LspI,NspV,SfuI  >ORV
  196. CviJI      2 rG'Cy          0 !                                    >
  197. CviRI      2 TG'CA          0 !                                    >
  198. ;CvnI      2 CC'TnA_GG      3 !  AxyI,Bsu36I,Eco81I,MstII,SauI     >BS
  199. DdeI       1 C'TnA_G        3 !                                    >BEILMNOPRUVX
  200. DpnI       2 GA'TC          0 !                                    >ABEILMNRSUVX
  201. ;DpnII     0 'GATC_         4 !  Bsp143I,MboI,NdeII,Sau3AI         >NU
  202. DraI       3 TTT'AAA        0 !                                    >ABEFIKLMNOPQRSUVX
  203. ;DraII     2 rG'GnC_Cy      3 !  EcoO109I,PssI                     >M
  204. DraIII     6 CAC_nnn'GTG   -3 !                                    >EMNSX
  205. DrdI       7 GACnn_nn'nnGTC  -2 !                                    >NU
  206. DrdII      3 GAACCA         ? !                                    >
  207. DsaI       1 C'CryG_G       4 !                                    >M
  208. ;DsaV      0 'CCnGG_        5 !  ScrFI                             >M
  209. EaeI       1 y'GGCC_r       4 !                                    >EKLMNSUVX
  210. EagI       1 C'GGCC_G       4 !  BstZI,EclXI,Eco52I,XmaIII         >NS
  211. ;Eam1104I   7 CTCTTCn'nnn_   3 !  EarI,Ksp632I                      >F
  212. Eam1105I   6 GACnn_n'nnGTC  -1 !                                    >FKN
  213. EarI       7 CTCTTCn'nnn_   3 !  Eam1104I,Ksp632I                  >NU
  214. EciI       3 TCCGCC         ? !                                    >
  215. ;Ecl136II   3 GAG'CTC        0 !  SacI,SstI                         >FN
  216. ;EclXI     1 C'GGCC_G       4 !  BstZI,EagI,Eco52I,XmaIII          >M
  217. ;Eco24I    5 G_rGCy'C      -4 !  BanII                             >F
  218. ;Eco31I    7 GGTCTCn'nnnn_   4 !  BsaI                              >F
  219. ;Eco32I    3 GAT'ATC        0 !  EcoRV                             >F
  220. ;Eco47I    1 G'GwC_C        3 !  AvaII,SinI                        >FOU
  221. Eco47III   3 AGC'GCT        0 !  Aor51HI                           >ABFLMNORU
  222. ;Eco52I    1 C'GGCC_G       4 !  BstZI,EagI,EclXI,XmaIII           >AFKORU
  223. Eco57I    22 CTGAAGnnnnnnnnnnnnnn_nn'  -2 !                                    >FN
  224. ;Eco64I    1 G'GyrC_C       4 !  BanI                              >F
  225. ;Eco72I    3 CAC'GTG        0 !  BbrPI,PmaCI,PmlI                  >F
  226. ;Eco81I    2 CC'TnA_GG      3 !  AxyI,Bsu36I,CvnI,MstII,SauI       >AFKOU
  227. ;Eco88I    1 C'yCGr_G       4 !  AvaI,BcoI,NspIII                  >F
  228. ;Eco91I    1 G'GTnAC_C      5 !  BstEII,BstPI,EcoO65I              >F
  229. ;Eco105I   3 TAC'GTA        0 !  SnaBI                             >FOU
  230. ;Eco130I   1 C'CwwG_G       4 !  EcoT14I,StyI                      >FU
  231. ;Eco147I   3 AGG'CCT        0 !  AatI,StuI                         >F
  232. EcoNI      5 CCTnn'n_nnAGG   1 !                                    >NSU
  233. ;EcoO65I   1 G'GTnAC_C      5 !  BstEII,BstPI,Eco91I               >AK
  234. EcoO109I   2 rG'GnC_Cy      3 !  DraII,PssI                        >AEFKLNSVX
  235. EcoRI      1 G'AATT_C       4 !                                    >ABEFHIKLMNOPQRSUVWX
  236. EcoRII     0 'CCwGG_        5 !  ApyI,BsiLI,BstNI,BstOI,MvaI       >BEOSUV
  237. EcoRV      3 GAT'ATC        0 !  Eco32I                            >ABEHIKLMNOPQRSUVWX
  238. ;EcoT14I   1 C'CwwG_G       4 !  Eco130I,StyI                      >AK
  239. ;EcoT22I   5 A_TGCA'T      -4 !  Mph1103I,NsiI,Ppu10I              >KOU
  240. ;EheI      3 GGC'GCC        0 !  BbeI,KasI,NarI                    >FOU
  241. ;EspI      2 GC'TnA_GC      3 !  Bpu1102I,CelII                    >U
  242. Esp3I      7 CGTCTCn'nnnn_   4 !                                    >FN
  243. FauI       9 CCCGCnnnn'nn_   2 !                                    >
  244. ;FbaI      1 T'GATC_A       4 !  BclI,BsiQI                        >K
  245. ;FdiII     3 TGC'GCA        0 !  AviII,FspI,MstI                   >U
  246. FinI       2 GTCCC          ? !                                    >
  247. Fnu4HI     2 GC'n_GC        1 !                                    >N
  248. ;FnuDII    2 CG'CG          0 !  AccII,Bsp50I,BstUI,MvnI,ThaI      >
  249. FokI      14 GGATGnnnnnnnnn'nnnn_   4 !                                    >AEIKMNRUVX
  250. FseI       6 GG_CCGG'CC    -4 !                                    >
  251. ;FsiI      1 r'AATT_y       4 !  ApoI                              >
  252. FspI       3 TGC'GCA        0 !  AviII,FdiII,MstI                  >NSU
  253. GdiII      1 y'GGCCG        6 !                                    >
  254. ;GsuI     22 CTGGAGnnnnnnnnnnnnnn_nn'  -2 !  BpmI                              >F
  255. HaeI       3 wGG'CCw        0 !                                    >
  256. HaeII      5 r_GCGC'y      -4 !  Bsp143II                          >ABEIKLMNOPRSUVX
  257. HaeIII     2 GG'CC          0 !  BshI,BsuRI,PalI                   >ABHIKLMNOPQRSUVX
  258. ;HapII     1 C'CG_G         2 !  HpaII,MspI                        >IK
  259. HgaI      10 GACGCnnnnn'nnnnn_   5 !                                    >NSUX
  260. HgiAI      5 G_wGCw'C      -4 !  Alw21I,AspHI,BsiHKAI              >NX
  261. ;HgiCI     1 G'GyrC_C       4 !  BanI,Eco64I                       >
  262. HgiEII     6 ACCnnnnnnGGT   ? !                                    >
  263. ;HgiJII    5 G_rGCy'C      -4 !  BanII,Eco24I                      >
  264. HhaI       3 G_CG'C        -2 !  CfoI,Hin6I,HinP1I                 >ABEKNOPRSUX
  265. ;Hin1I     2 Gr'CG_yC       2 !  AcyI,BbiII,BsaHI                  >EFOU
  266. ;Hin6I     1 G'CG_C         2 !  CfoI,HhaI,HinP1I                  >F
  267. ;HinP1I    1 G'CG_C         2 !  CfoI,HhaI,Hin6I                   >NSX
  268. HincII     3 GTy'rAC        0 !  HindII                            >ABFHIKLNOPQRSUVX
  269. ;HindII    3 GTy'rAC        0 !  HincII                            >EM
  270. HindIII    1 A'AGCT_T       4 !                                    >ABEFHIKLMNOPQRSUVWX
  271. HinfI      1 G'AnT_C        3 !                                    >ABEFHIKLMNOPQRSUVWX
  272. HpaI       3 GTT'AAC        0 !                                    >ABEFHIKLMNOPQRSUVX
  273. ;HpaII     1 C'CG_G         2 !  HapII,MspI                        >ABEFLMNOPQRSUVX
  274. HphI      13 GGTGAnnnnnnn_n'  -1 !                                    >NSUVX
  275. ;KasI      1 G'GCGC_C       4 !  BbeI,EheI,NarI                    >N
  276. KpnI       5 G_GTAC'C      -4 !  Acc65I,Asp718I                    >ABEFHIKLMNOPQRSUVWX
  277. ;Kpn2I     1 T'CCGG_A       4 !  AccIII,BseAI,BsiMI,BspEI,MroI     >F
  278. ;KspI      4 CC_GC'GG      -2 !  Cfr42I,SacII,SstII                >M
  279. ;Ksp632I   7 CTCTTCn'nnn_   3 !  Eam1104I,EarI                     >M
  280. ;LspI      2 TT'CG_AA       2 !  BsiCI,Bsp119I,BstBI,Csp45I,NspV,SfuI  >L
  281. ;MaeI      1 C'TA_G         2 !  BfaI,RmaI                         >M
  282. MaeII      1 A'CG_T         2 !                                    >M
  283. MaeIII     0 'GTnAC_        5 !                                    >M
  284. ;MamI      5 GATnn'nnATC    0 !  BsaBI,BsiBI                       >M
  285. ;MboI      0 'GATC_         4 !  Bsp143I,DpnII,NdeII,Sau3AI        >BEFIKNPQRSVX
  286. MboII     13 GAAGAnnnnnnn_n'  -1 !                                    >BFIKNOPQRSUVX
  287. ;McrI      4 CG_ry'CG      -2 !  BsiEI                             >M
  288. ;MfeI      1 C'AATT_G       4 !  MunI                              >
  289. ;MflI      1 r'GATC_y       4 !  BstYI,XhoII                       >AK
  290. MluI       1 A'CGCG_T       4 !                                    >ABEFIKLMNOPQRSUVWX
  291. MlyI      10 GACTCnnnnn'    0 !                                    >
  292. MmeI      26 TCCrACnnnnnnnnnnnnnnnnnn_nn'  -2 !                                    >
  293. MnlI      11 CCTCnnnnnn_n'  -1 !                                    >ENQSUX
  294. ;Mph1103I   5 A_TGCA'T      -4 !  EcoT22I,NsiI,Ppu10I               >F
  295. ;MroI      1 T'CCGG_A       4 !  AccIII,BseAI,BsiMI,BspEI,Kpn2I    >MOU
  296. MscI       3 TGG'CCA        0 !  BalI                              >BNSU
  297. MseI       1 T'TA_A         2 !                                    >NSU
  298. MslI       5 CAynnnnrTG     ? !                                    >
  299. MspI       1 C'CG_G         2 !  HapII,HpaII                       >ABEFHIKLMNOPQRSUVWX
  300. ;MstI      3 TGC'GCA        0 !  AviII,FdiII,FspI                  >X
  301. ;MstII     2 CC'TnA_GG      3 !  AxyI,Bsu36I,CvnI,Eco81I,SauI      >EX
  302. MunI       1 C'AATT_G       4 !                                    >BFN
  303. ;MvaI      2 CC'w_GG        1 !  ApyI,BsiLI,BstNI,BstOI,EcoRII     >AFKMOU
  304. ;MvnI      2 CG'CG          0 !  AccII,Bsp50I,BstUI,ThaI           >M
  305. ;MwoI      7 GCnn_nnn'nnGC  -3 !  BspWI                             >
  306. NaeI       3 GCC'GGC        0 !  NgoMI                             >AEKLMNOUVX
  307. NarI       2 GG'CG_CC       2 !  BbeI,EheI,KasI                    >BEMNOPRSUVX
  308. NciI       2 CC's_GG        1 !  BcnI                              >BELMNOSUVWX
  309. NcoI       1 C'CATG_G       4 !                                    >ABEFHIKLMNOPQRSUVWX
  310. NdeI       2 CA'TA_TG       2 !                                    >BEFKLMNPSUVX
  311. ;NdeII     0 'GATC_         4 !  Bsp143I,DpnII,MboI,Sau3AI         >BM
  312. ;NgoMI     1 G'CCGG_C       4 !  NaeI                              >N
  313. NheI       1 G'CTAG_C       4 !                                    >ABEKLMNOPRSUVX
  314. NlaIII     4 _CATG'        -4 !                                    >NSU
  315. NlaIV      3 GGn'nCC        0 !  BscBI                             >NSU
  316. NotI       2 GC'GGCC_GC     4 !                                    >ABEFIKLMNOPQRSUVWX
  317. NruI       3 TCG'CGA        0 !  Bsp68I,SpoI                       >ABEIKLMNOPQSUVWX
  318. NsiI       5 A_TGCA'T      -4 !  EcoT22I,Mph1103I,Ppu10I           >BELMNRSVX
  319. NspI       5 r_CATG'y      -4 !                                    >AKMU
  320. ;NspIII    1 C'yCGr_G       4 !  AvaI,BcoI,Eco88I                  >P
  321. NspV       2 TT'CG_AA       2 !  BsiCI,Bsp119I,BstBI,Csp45I,LspI,SfuI  >ABKOP
  322. NspBII     3 CmG'CkG        0 !                                    >U
  323. PacI       5 TTA_AT'TAA    -2 !                                    >NU
  324. ;PaeI      5 G_CATG'C      -4 !  BbuI,SphI                         >F
  325. ;PaeR7I    1 C'TCGA_G       4 !  CcrI,XhoI                         >NSX
  326. ;PalI      2 GG'CC          0 !  BshI,BsuRI,HaeIII                 >EPV
  327. ;Pfl23II   1 C'GTAC_G       4 !  BsiWI,SplI,SunI                   >F
  328. Pfl1108I   3 TCGTAG         ? !                                    >
  329. PflMI      7 CCAn_nnn'nTGG  -3 !  Van91I                            >NSU
  330. PleI       9 GAGTCnnnn'n_   1 !                                    >NU
  331. ;PmaCI     3 CAC'GTG        0 !  BbrPI,Eco72I,PmlI                 >AK
  332. PmeI       4 GTTT'AAAC      0 !                                    >N
  333. PmlI       3 CAC'GTG        0 !  BbrPI,Eco72I,PmaCI                >NU
  334. ;Ppu10I    1 A'TGCA_T       4 !  EcoT22I,Mph1103I,NsiI             >F
  335. ;PpuMI     2 rG'GwC_Cy      3 !  Psp5II                            >NSU
  336. PshAI      5 GACnn'nnGTC    0 !                                    >K
  337. Psp5II     2 rG'GwC_Cy      3 !  PpuMI                             >BF
  338. ;PspAI     1 C'CCGG_G       4 !  Cfr9I,SmaI,XmaI                   >E
  339. ;PssI      5 rG_GnC'Cy     -3 !  DraII,EcoO109I                    >I
  340. PstI       5 C_TGCA'G      -4 !                                    >ABEFHIKLMNOPQRSUVWX
  341. PvuI       4 CG_AT'CG      -2 !  BspCI,XorII                       >ABEFKLMNOPQRSUVX
  342. PvuII      3 CAG'CTG        0 !                                    >ABEFIKLMNOPQRSUVWX
  343. RleAI     18 CCCACAnnnnnnnnn_nnn'  -3 !                                    >
  344. ;RmaI      1 C'TA_G         2 !  BfaI,MaeI                         >U
  345. RsaI       2 GT'AC          0 !  AfaI,Csp6I                        >ABEILMNOPQRSUVWX
  346. RsrII      2 CG'GwC_CG      3 !  CpoI,CspI                         >ABMNSUX
  347. SacI       5 G_AGCT'C      -4 !  Ecl136II,SstI                     >AEIKLMNOPQRSUVWX
  348. SacII      4 CC_GC'GG      -2 !  Cfr42I,KspI,SstII                 >EILNOPQRSUVX
  349. SalI       1 G'TCGA_C       4 !                                    >ABEFHIKLMNOPQRSUVWX
  350. SapI       8 GCTCTTCn'nnn_   3 !                                    >
  351. ;SauI      2 CC'TnA_GG      3 !  AxyI,Bsu36I,CvnI,Eco81I,MstII     >M
  352. Sau96I     1 G'GnC_C        3 !  AsuI,BsiZI,Cfr13I                 >BELMNORSVX
  353. Sau3AI     0 'GATC_         4 !  Bsp143I,DpnII,MboI,NdeII          >ABEHIKLMNOPQRSUVX
  354. ScaI       3 AGT'ACT        0 !                                    >ABEFIKLMNOPQRSUVX
  355. ScrFI      2 CC'n_GG        1 !  DsaV                              >EMNOSUVX
  356. ;SduI      5 G_dGCh'C      -4 !  BmyI,Bsp1286I                     >F
  357. ;SecI      1 C'CnnG_G       4 !  BsaJI                             >
  358. SfaNI     10 GCATCnnnnn'nnnn_   4 !                                    >NSUX
  359. SfcI       1 C'TryA_G       4 !                                    >N
  360. ;SfeI      1 C'TryA_G       4 !  SfcI                              >
  361. SfiI       8 GGCCn_nnn'nGGCC  -3 !                                    >ABEILMNOPQRSUVX
  362. ;SfuI      2 TT'CG_AA       2 !  BsiCI,Bsp119I,BstBI,Csp45I,LspI,NspV  >M
  363. SgrAI      2 Cr'CCGG_yG     4 !                                    >M
  364. ;SinI      1 G'GwC_C        3 !  AvaII,Eco47I                      >LRSV
  365. SmaI       3 CCC'GGG        0 !  Cfr9I,PspAI,XmaI                  >ABEFHIKLMNOPQRSUVWX
  366. ;SnaI      3 GTATAC         ? !  Bst1107I                          >
  367. SnaBI      3 TAC'GTA        0 !  Eco105I                           >AEKLMNRSVX
  368. ;SnoI      1 G'TGCA_C       4 !  Alw44I,ApaLI                      >LMV
  369. SpeI       1 A'CTAG_T       4 !                                    >ABEKLMNORSUVWX
  370. SphI       5 G_CATG'C      -4 !  BbuI,PaeI                         >ABEHIKLMNOPQRSUX
  371. ;SplI      1 C'GTAC_G       4 !  BsiWI,Pfl23II,SunI                >AK
  372. ;SpoI      3 TCG'CGA        0 !  Bsp68I,NruI                       >R
  373. SrfI       4 GCCC'GGGC      0 !                                    >E
  374. Sse8387I   6 CC_TGCA'GG    -4 !                                    >AK
  375. SspI       3 AAT'ATT        0 !                                    >ABEFKLMNORUVX
  376. ;SstI      5 G_AGCT'C      -4 !  Ecl136II,SacI                     >BS
  377. ;SstII     4 CC_GC'GG      -2 !  Cfr42I,KspI,SacII                 >BS
  378. StuI       3 AGG'CCT        0 !  AatI,Eco147I                      >ABEIKLMNPQRSVX
  379. StyI       1 C'CwwG_G       4 !  Eco130I,EcoT14I                   >BEMNRSUVX
  380. SunI       1 C'GTAC_G       4 !  BsiWI,Pfl23II,SplI                >B
  381. SwaI       4 ATTT'AAAT      0 !                                    >M
  382. TaqI       1 T'CG_A         2 !  TthHB8I                           >ABEFILMNOPQRSUVWX
  383. TaqII-1   17 GACCGAnnnnnnnnn_nn'  -2 !                                    >
  384. TaqII-2   17 CACCCAnnnnnnnnn_nn'  -2 !                                    >
  385. TfiI       1 G'AwT_C        3 !                                    >NU
  386. ThaI       2 CG'CG          0 !  AccII,Bsp50I,BstUI,MvnI           >BI
  387. Tsp45I     2 GTsAC          ? !                                    >
  388. TspEI      2 AATT           ? !                                    >
  389. Tth111I    4 GACn'n_nGTC    1 !  AspI                              >AEIKNPQRUVX
  390. Tth111II  17 CAArCAnnnnnnnnn_nn'  -2 !                                    >
  391. ;TthHB8I   1 T'CG_A         2 !  TaqI                              >AK
  392. ;Van91I    7 CCAn_nnn'nTGG  -3 !  PflMI                             >FM
  393. ;VspI      2 AT'TA_AT       2 !  AseI,AsnI                         >FKR
  394. XbaI       1 T'CTAG_A       4 !                                    >ABEFHIKLMNOPQRSUVWX
  395. XcmI       8 CCAnnnn_n'nnnnTGG  -1 !                                    >NU
  396. XhoI       1 C'TCGA_G       4 !  CcrI,PaeR7I                       >ABEFHIKLMNOPQRSUVWX
  397. ;XhoII     1 r'GATC_y       4 !  BstYI,MflI                        >EMRVX
  398. ;XmaI      1 C'CCGG_G       4 !  Cfr9I,PspAI,SmaI                  >INRSUVWX
  399. ;XmaIII    1 C'GGCC_G       4 !  BstZI,EagI,EclXI,Eco52I           >BE
  400. XmnI       5 GAAnn'nnTTC    0 !  Asp700I                           >ENX
  401. ;XorII     4 CG_AT'CG      -2 !  BspCI,PvuI                        >BS
  402.  
  403.